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High-throughput Fluorescence Cell Counter
To accurately evaluate the viability of cell types like PBMCs, stem cells, and splenocytes, it is advisable to use dual-fluorescence staining with high throughput fluorescence cell counter.
AO (Acridine Orange) and DAPI (4′, 6-diamidino-2-phenylindole) are fluorescent dyes that bind to DNA, staining the nucleus of targeted cells.
AO is a permeable dye that stains the nucleus of all cells, regardless of the cell’s condition.
DAPI is an impermeable dye that only stains the nucleus of non-viable cells with damaged membranes.
This method effectively excludes non-nucleated cells (e.g., RBCs), platelets, and debris from the samples.
AO/DAPI staining - Counting & Viability
Accurate Fluorescent Cell Counting for PBMCs
PBMCs are often mixed with RBCs or platelets. Use EVE™ HT FL to count only nucleated cells such as PBMCs.
As a specialized high throughput fluorescence cell counter, its fluorescence-based counting is more accurate than traditional Trypan Blue-based counting.
More Flexible Staining Methods
EVE™ HT FL provides flexible and personalized staining choices. Cell viability can be assessed using fluorescent channels (AO/DAPI) or a bright-field channel (Trypan Blue / Erythrosine B), meeting a more diverse range of needs in high throughput cell counting.
● A Growing Demand for Using Erythrosine B
Erythrosine B has the same staining principle as Trypan Blue which does not penetrate viable cells that possess intact cell membranes but only stains non-viable cells. Due to increasing environmental and health concerns, non-toxic Erythrosine B has gained attention in many European countries and is regarded as an alternative to Trypan Blue for cell viability evaluation.
Up to 48 Samples in 3 minutes
Don’t wait! Achieve a rapid high throughput cell count in just 3 minutes.
This walk away cell counter leads to substantial time saving by eliminating the need for frequent reloading and waiting periods.
20 μL of sample volume
EVE™ HT FL only needs 20 μL of your valuable samples for high throughput cell analysis.
Save your cells for more measurements or better outcomes.
Level Up Your Productivity
Level up your productivity with EVE™ HT FL, the premier automated high throughput cell counting system, when compared to other cell counters.
● Multi-Instrument Consistency & Precision for Cells
EVE™ HT FL instruments were put to the test using three different cell line samples (Jurkat, HeLa, and CHO) to compare their differences.
The result below showed a high instrument-to-instrument consistency with small variation, proving its reliability as an automated high throughput cell counter.
● Excellent Linearity Across Wide Range
Following ISO standard for cell counting, we evaluated linearity of EVE™ HT FL using 2 cell lines (CHO cells and Jurkat cells).
The following results demonstrate outstanding linearity.
● High correlation between EVE™ HT FL and flow cytometer
Cell samples were measured using EVE™ HT FL and a flow cytometer.
For both CHO cells and PBMCs, total cell counts measured by EVE™ HT FL were highly correlated with those measured by a flow cytometer.
EVE™ HT FL is 21 CFR Part 11 compliant, making it a fully compliant automated high throughput cell counter for cGMP facilities.
With visualized data, the results are clear at a glance.
(Total cell, Live/Dead Cell, Viability, Cell Size)
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Product name / Cat. No.
EVE™ HT FL / EVE–HT FL
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Light sources
Bright field, UV, Blue LED
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Staining dye(s)
AO/DAPI mixed solution
Trypan blue stain
Erythrosin B stain -
Analysis time
3–20 minutes / 48 tests
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Measuring range
Detectable range: 1 x 10E4 – 2 x 10E7 cells/mL
Optimal range: 1 x 10E5 – 1 x 10E7 cells/mL -
Loading volume
20 μL / channel
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Cell size range
Detectable size: 5–85 μm
Optimal size: 5–80 μm -
Dimensions
586 (W) x 461 (D) x 458 (H) mm
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Weight
61 kg
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Operation system
Windows 10
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21 CFR Part 11 compliance
Available (Optional)
Related downloads Total 3
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EVE HT FL_Brochure_NanoEntek
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EVE HT FL_Manual_NanoEntek
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EVE™ HT FL Poster_A new high-throughput fluorescence cell counter
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